Preparation of human rabies vaccine in VERO cell culture using a microcarrier system
Braz. j. med. biol. res
; 26(12): 1305-17, Dec. 1993. ilus, tab, graf
Article
in En
| LILACS
| ID: lil-148836
Responsible library:
BR1.1
ABSTRACT
1. The rabies virus (Pasteur PV strain) was propagated in VERO cells attached to microcarriers in a 3.7-1 bioreactor. Virus titers of about 10(6) LD50/ml were obtained regularly. 2. Ultrafiltration was efficient for concentrating the virus suspensions, and the sucrose gradient reduced the residual VERO cell DNA to acceptable levels (less than 50 pg/dose). The remaining cell DNA content was evaluated by dot-blot hybridization with a probe prepared with VERO cell DNA. 3. The final virus preparations were inactivated by B-propiolactone treatment, showed a potency higher than 2.5 IU/dose and protected mice experimentally infected intracerebrally with rabies virus (CVS-13.2). 4. This methodology for the production of a rabies vaccine for human use should be of interest to countries where high technology facilities are not available
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Collection:
01-internacional
Database:
LILACS
Main subject:
Rabies virus
/
Rabies Vaccines
/
Antibodies, Viral
Limits:
Animals
/
Humans
Language:
En
Journal:
Braz. j. med. biol. res
Journal subject:
BIOLOGIA
/
MEDICINA
Year:
1993
Document type:
Article
Country of publication:
Brazil