Characterization of clinical isolates of Streptococcus agalactiae by random amplified polymorphic DNA using degenerate oligonucleotides
Medicina (B.Aires)
; 55(6): 681-4, 1995. tab, ilus
Article
in English
| LILACS
| ID: lil-163814
Responsible library:
BR1.1
RESUMO
Epidemioiogicai studies of Streptococcus agalactiae strains have been limited by the lack of sensitive and discriminatory methods for comparing clinical isolates. Serotyping, albeit a widely used methodology, has been shown to possess low capability to distinguish between epidemiologically related and unrelated isolates. We have employed here a random amplification of polymorphic DNA (RAPD) assay, using degenerate oligonucleotides as primers, to characterize S. agalactiae isolates from related or unrelated clinical samples. Epidemioiogically-related isolates (mother-infant pairs) showed identical profiles by this methodology. On the contrary, 12 epidemioiogically-unrelated isolates (ciassified into 5 different serotypes) resulted in ll distinct RAPD patterns. This suggests that the proposed modified RAPD assay provides a highly discriminatory tool for the analysis of genomic diversity among isolates from pathogenic organisms.
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Collection:
International databases
Database:
LILACS
Main subject:
Streptococcus agalactiae
/
Random Amplified Polymorphic DNA Technique
Type of study:
Controlled clinical trial
Limits:
Female
/
Humans
/
Infant, Newborn
/
Pregnancy
Language:
English
Journal:
Medicina (B.Aires)
Journal subject:
Medicine
Year:
1995
Document type:
Article