Extraction, purification and some partial characterization of Ó-amylase inhibitors from wheat lapar 28-igapó / Extracción, purificación y algunas características parciales de los inhibidores de Ó-amilasa del trigo 28-igapó

RESUMEN

Ó-Amylase inhibitors wheat (Triticum aestivum) cultivar lapar 28-Igapó were extrated with water in a 110 (wv) ratio and precipitated with ammonium sulfate between 20-20 por ciento saturation, followed by DEAE and CM-cellulose chromatography. One inhibitor was purified and designated as CMC-IB, and had electrophoretic mobilities of 0.23 and 0.54 in alkaline and acidic conditions, respectively. This inhibitor was 750 times more active on human salivary Ó-amylase (HSSA) than porcine pancreatic Õ-amylase (PPA). The preincubation time required for maximum complexation with HSA was 20 minutes and optimum pH of inhibition was 7.5. The inhibitor CMC-IB was stable at 0ºC and maintained 50 por ciento of inhibotory activity against HSA, when incubated at 98ºC for one hour