The Amazonia variant of Vibrio cholerae: molecular identification and study of virulence genes
Mem. Inst. Oswaldo Cruz
; 93(5): 601-7, Sept.-Oct. 1998. ilus
Article
in English
| LILACS
| ID: lil-217854
Responsible library:
BR15.1
RESUMO
The pathogenic O1 Amazonia variant of Vibrio cholerae has been shown previously to have a cytotoxin acting on cultured Vero and Y-1 cells, and to lack important virulence factors such as the cholera toxin (Coelho et al. 1995a). This study extends the molecular analysis of the Amazonia strains, detecting the presence to the toxR gene, with a very similar sequence to that of the El Tor and classical biotypes. The outer membrane proteins are analysed, detecting a variation among the group of Amazonia strains, with three different patterns found. As a by-product of this work a polymerase chain reation fragment was sequenced, reading part of the sequence of the Lon protease of the Amazonia strains. The gene was not previously described in V. cholerae, but its sequences is present in the TIGR database specific for this species.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Vibrio cholerae
/
Virulence
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1998
Document type:
Article
/
Congress and conference