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Regulation of T cell response to leishmania antigens by determinants of histocompatibility leukocyte class I and II molecules
Bacellar, O; Russo, C; Carvalho, E. M.
Affiliation
  • Bacellar, O; Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia.
  • Russo, C; Cornell University Graduate School of Medical Science. Division of International Medicine and Infectious Diseases.
  • Carvalho, E. M; Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia.
Braz. j. med. biol. res ; 31(12): 1575-81, Dec. 1998. tab, graf
Article in English | LILACS | ID: lil-224843
Responsible library: BR1.1
ABSTRACT
It has been shown that HLA class I molecules play a significant role in the regulation of the proliferation of T cells activated by mitogens and antigens. We evaluated the ability of mAb to a framework determinant of HLA class I molecules to regulate T cell proliferation and interferon gamma (IFN-FACE="Symbol">g) production against leishmania, PPD, C. albicans and tetanus toxoid antigens in patients with tegumentary leishmaniasis and healthy subjects. The anti-major histocompatibility complex (MHC) mAb (W6/32) suppressed lymphocyte proliferation by 90 percent in cultures stimulated with FACE="Symbol">aCD3, but the suppression was variable in cultures stimulated with leishmania antigen. This suppression ranged from 30-67 percent and was observed only in 5 of 11 patients. IFN-FACE="Symbol">g production against leishmania antigen was also suppressed by anti-HLA class I mAb. In 3 patients IFN-FACE="Symbol">g levels were suppressed by more than 60 percent, while in the other 2 cultures IFN-FACE="Symbol">g levels were 36 and 10 percent lower than controls. The suppression by HLA class I mAb to the proliferative response in leishmaniasis patients and in healthy controls varied with the antigens and the patients or donors tested. To determine whether the suppression is directed at antigen presenting cells (APCs) or at the responding T cells, experiments with antigen-primed non-adherent cells, separately incubated with W6/32, were performed. Suppression of proliferation was only observed when the W6/32 mAb was added in the presence of T cells. These data provide evidence that a mAb directed at HLA class I framework determinants can suppress proliferation and cytokine secretion in response to several antigens
Subject(s)
Full text: Available Collection: International databases Database: LILACS Main subject: T-Lymphocytes / Interferon-gamma / Leishmaniasis, Cutaneous / HLA Antigens / Leishmania / Antibodies, Monoclonal / Antigens, Protozoan Limits: Animals / Humans Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 1998 Document type: Article
Full text: Available Collection: International databases Database: LILACS Main subject: T-Lymphocytes / Interferon-gamma / Leishmaniasis, Cutaneous / HLA Antigens / Leishmania / Antibodies, Monoclonal / Antigens, Protozoan Limits: Animals / Humans Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 1998 Document type: Article
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