Use of remazol blue dyed avicel for the determination of cellullolytic activity in basidiomycetes
Rev. microbiol
; 29(4): 286-8, out.-dez. 1998. graf
Article
in English
| LILACS
| ID: lil-251738
Responsible library:
BR32.1
ABSTRACT
A modified method for direct determination of cellulolytic activity using Avicel colored with Remazol Brilliant Blue R (RBBR) in Agar test tubes in discussed. Refinaments were introduced in a simple method for qunatitation of cellulase activity, based on the release of dye from Avicel-RBBR medium by enzimatic hydrolysis. Modification in Avicel-dye preparation were enhanced and a spectrophotometer improved the precision of the collected data, since absorbance measurements could be done at the maxmum wavelenght for RBBR (595 nm).
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Basidiomycota
/
In Vitro Techniques
/
Coloring Agents
Language:
English
Journal:
Rev. microbiol
Journal subject:
Microbiology
Year:
1998
Document type:
Article
Affiliation country:
Brazil