Cáncer gástrico y regulación del crecimiento tumoral: estudio de marcadores de proliferación celular y del complejo proteínas p53/p21WAF1/CIP1/mdm-2 / Gastric cancer and tumor growth regulation: study of cell proliferation markers and protein complex p53/p21WAF1/CIP1/mdm-2
Rev. méd. Chile
; 128(2): 127-36, feb. 2000. ilus, tab
Article
in Spanish
| LILACS
| ID: lil-258109
Responsible library:
CL1.1
RESUMO
Gastric cancer and tumor growth regulation. Study of cell proliferation markers and protein complex p53/p21WAF1/CIP1/mdm-2. Background:
p53/p21WAF1/CIP1 and mdm-2 proteins play an important role in cell cycle regulation. The study of the pathogenesis of gastric cancer is important to understand how these tumors progress during their natural history.Aim:
To study the relationship between the immunohistochemical expression of p53/p21WAF1/CIP1 and mdm-2 and cell proliferation in gastric cancer. Material andmethods:
Fifty one gastrectomy specimens with gastric cancer were studied using immunohistochemistry for p53/p21WAF1/CIP1 and mdm-2. Cell proliferation was determined by immunolabelling with PCNA and Ki67 antigens. Mitosis figures were counted in 10 high power microscopic fields.Results:
Patients from whom gastric cancer specimens were obtained had a mean age of 59.3 years. Ki67 and mitosis counting showed the highest correlation index with proliferation indexes studied. No correlation was found between the expression of protein complex p53/p21WAF1/CIP1 and mdm-2 and morphological characteristics of gastric tumors. Mdm-2 protein overexpression was the only marker that showed an independent correlation with cell proliferation. Moreover, mdm-2 positive tumors showed the highest proliferation indexes when p53 was not immunohistochemically over-expressed, as determined by PCNA labelling index.Conclusions:
In gastric cancer, a direct correlation between mdm-2 overexpression and cell proliferation was observed. Moreover, the fact that mdm-2 positive tumors showed the highest cell proliferation when p53 was not overexpressed, entitles us to hypothesize that mdm-2 overexpression could play a major role in gastric carcinogenesis
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Stomach Neoplasms
/
Biomarkers, Tumor
/
Neoplasm Proteins
Limits:
Female
/
Humans
/
Male
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2000
Document type:
Article
/
Project document