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An in vivo method for estimating the concentration of crotamine in a solution
Hampe, O. G; Belló, A. A.
Affiliation
  • Hampe, O. G; Universidade Federal do Rio Grande do Sul. Instituto de Biociências.
  • Belló, A. A; Universidade Federal do Rio Grande do Sul. Instituto de Biociências.
J. venom. anim. toxins ; 3(1): 33-6, 1997. ilus
Article in English | LILACS | ID: lil-276619
Responsible library: BR33.1
RESUMO
A very sensitive method for estimating the concentration of crotamine in a solution was developed. This method was based on the based on the time required for the appearance of permanent hyperextension of the rear legs of mice as a function of the dose administered. This method can be used to determine toxin doses as low as 0.32 mg/kg(-1). Its high specificity for crotamine means that it can be used to measure toxin concentrations in the presence of other proteins and polypeptides.
Subject(s)
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Full text: Available Collection: International databases Database: LILACS Main subject: Crotalus / Crotalid Venoms Limits: Animals Language: English Journal: J. venom. anim. toxins Journal subject: Toxicology Year: 1997 Document type: Article
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Full text: Available Collection: International databases Database: LILACS Main subject: Crotalus / Crotalid Venoms Limits: Animals Language: English Journal: J. venom. anim. toxins Journal subject: Toxicology Year: 1997 Document type: Article