Catecholamine-induced vasoconstriction is sensitive to carbonic anhydrase I activation
Braz. j. med. biol. res
; 34(3): 339-345, Mar. 2001. ilus, tab
Article
in English
| LILACS
| ID: lil-281614
Responsible library:
BR1.1
RESUMO
We studied the relationship between alpha- and beta-adrenergic agonists and the activity of carbonic anhydrase I and II in erythrocyte, clinical and vessel studies. Kinetic studies were performed. Adrenergic agonists increased erythrocyte carbonic anhydrase as follows adrenaline by 75 percent, noradrenaline by 68 percent, isoprenaline by 55 percent, and orciprenaline by 62 percent. The kinetic data indicated a non-competitive mechanism of action. In clinical studies carbonic anhydrase I from erythrocytes increased by 87 percent after noradrenaline administration, by 71 percent after orciprenaline and by 82 percent after isoprenaline. The increase in carbonic anhydrase I paralleled the increase in blood pressure. Similar results were obtained in vessel studies on piglet vascular smooth muscle. We believe that adrenergic agonists may have a dual mechanism of action the first one consists of a catecholamine action on its receptor with the formation of a stimulus-receptor complex. The second mechanism proposed completes the first one. By this second component of the mechanism, the same stimulus directly acts on the carbonic anhydrase I isozyme (that might be functionally coupled with adrenergic receptors), so that its activation ensures an adequate pH for stimulus-receptor coupling for signal transduction into the cell, resulting in vasoconstriction
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Collection:
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Database:
LILACS
Main subject:
Vasoconstriction
/
Catecholamines
/
Carbonic Anhydrases
/
Adrenergic alpha-Agonists
/
Adrenergic beta-Agonists
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2001
Document type:
Article
Affiliation country:
Germany
/
Romania
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United States
Institution/Affiliation country:
Faculty of Medicine/RO
/
Klinik Wilkenberg/DE
/
Medical Care Unit/US
/
Simleu Silvaniei/RO