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Serodiagnosis of chronic Chagas infection by using EIE-Recombinant-Chagas-Biomanguinhos kit
Gomes, Yara M; Pereira, Valéria R. A; Nakazawa, Mineo; Rosa, Daniela S; Barros, Maria das Neves D. S; Ferreira, Antonio G. P; Silva, Edimilson D; Ogatta, Sueli F Yamada; Krieger, Marco Aurélio; Goldenberg, Samuel.
Affiliation
  • Gomes, Yara M; Fiocruz. Centro de Pesquisas Aggeu Magalhäes. Departamento de Imunologia. Recife. BR
  • Pereira, Valéria R. A; Fiocruz. Centro de Pesquisas Aggeu Magalhäes. Departamento de Imunologia. Recife. BR
  • Nakazawa, Mineo; Fiocruz. Centro de Pesquisas Aggeu Magalhäes. Departamento de Imunologia. Recife. BR
  • Rosa, Daniela S; Fiocruz. Centro de Pesquisas Aggeu Magalhäes. Departamento de Imunologia. Recife. BR
  • Barros, Maria das Neves D. S; UPE. Hospital Universitário Oswaldo Cruz. Ambulatório de Doença de Chagas. Recife. BR
  • Ferreira, Antonio G. P; Fiocruz. Bio-Manguinhos. Instituto de Tecnologia em Imunobiológicos. Laboratório de Reativos. Rio de Janeiro. BR
  • Silva, Edimilson D; Fiocruz. Bio-Manguinhos. Instituto de Tecnologia em Imunobiológicos. Laboratório de Reativos. Rio de Janeiro. BR
  • Ogatta, Sueli F Yamada; Universidade Estadual de Londrina. Departamento de Microbiologia. Londrina. BR
  • Krieger, Marco Aurélio; Fiocruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro. BR
  • Goldenberg, Samuel; Fiocruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro. BR
Mem. Inst. Oswaldo Cruz ; 96(4): 497-501, May 2001. ilus, tab
Article in En | LILACS | ID: lil-285556
Responsible library: BR1.1
ABSTRACT
A kit based on an enzyme immunoassay, EIE-Recombinant-Chagas-Biomanguinhos, developed by the Oswaldo Cruz Foundation, was evaluated for the serodiagnosis of chronic Chagas disease. Evaluation was performed with 368 serum samples collected from individuals living in an endemic area for Chagas disease 131 patients in the chronic phase with confirmed clinical, epidemiological, and serological diagnosis (indirect immunofluorescence, indirect hemagglutination or enzyme-linked immunosorbent assay) and 237 nonchagasic seronegative individuals were considered negative control. The EIE-Recombinant-Chagas-Biomanguinhos kit showed high sensitivity, 100 percent (CI 95 percent 96.4-100 percent) and high specificity, 100 percent (CI 95 percent 98-100 percent). The data obtained were in full agreement with clinical and conventional serology data. In addition, no cross-reaction was observed with sera from patients with cutaneous (n=14) and visceral (n=3) leishmaniasis. However, when these sera were tested by conventional serological assays for Chagas disease, cross-reactions were detected in 14.3 percent and 33.3 percent of the patients with cutaneous and visceral leishmaniasis, respectively. No cross-reactions were observed when sera from nonchagasic seronegative patients bearing other infectious disease (syphilis, n=8; HTLV, n=8; HCV, n=7 and HBV, n=12) were tested. In addition, sera of patients with inconclusive results for Chagas disease by conventional serology showed results in agreement with clinical evaluation, when tested by the kit. These results are relevant and indicate that the refered kit provides a safe immunodiagnosis of Chagas disease and could be used in blood bank screening
Subject(s)
Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Recombinant Proteins / Chagas Disease / Antigens, Protozoan Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Journal: Mem. Inst. Oswaldo Cruz Journal subject: MEDICINA TROPICAL / PARASITOLOGIA Year: 2001 Document type: Article Affiliation country: Brazil Country of publication: Brazil
Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Recombinant Proteins / Chagas Disease / Antigens, Protozoan Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Journal: Mem. Inst. Oswaldo Cruz Journal subject: MEDICINA TROPICAL / PARASITOLOGIA Year: 2001 Document type: Article Affiliation country: Brazil Country of publication: Brazil