Evaluation of an ELISA for detection of antibodies to Babesia bigemina in cattle and it's application in an epidemiological survey in Brazil
Pesqui. vet. bras
; 21(2): 72-6, abr.-jun. 2001. ilus, tab
Article
in En
| LILACS
| ID: lil-290514
Responsible library:
BR68.1
RESUMO
An indirect enzyme-linked immunosorbent assay (ELISA) using a crude antigen was evaluated for its performance to detect Babesia bigemina antibodies. The sensitivity and specificity were 98.0 per cent and 99.0 per cent, respectively. In agreement with the high specificity, no cross-reactions were verified with sera from calves inoculated three times with 10 7(subscribe) Babesia bovis organisms. With regard to the comparison of ELISA and indirect fluorescent antibody test (IFAT) in detecting antibodies against B. bigemina in calves experimentally infected with five Brazilian geographical isolates of this hemoparasite, IFAT was able to detect antibodies one day earlier in most of the calves' sera. There was a good agreement between results shown by ELISA and IFAT with sera from an enzootically stable area (k=0.61). However, there was no agreement between these serological tests with sera from an enzootically unstable area (k=0.33). The ELISA was employed in an epidemiological survey using with 1,367 sera from four counties in the Pantanal of Mato Grosso do Sul and characterized this region as an enzootically stable area, since the prevalence ranged from 87.7 to 98.9 per cent. Therefore, this ELISA with high sensitivity, specificity and performance similar to IFAT can be employed in serological diagnosis of B. bigemina
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Babesia
/
Enzyme-Linked Immunosorbent Assay
/
Serologic Tests
Type of study:
Diagnostic_studies
/
Risk_factors_studies
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Pesqui. vet. bras
Journal subject:
MEDICINA VETERINARIA
Year:
2001
Document type:
Article
Affiliation country:
Brazil
Country of publication:
Brazil