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Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cassia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens
Bragazza, Lúcia M; Vaz, Adelaide J; Passos, Afonso D. C; Takayanagui, Osvaldo M; Nakamura, Paulo M; Espíndola, Noeli M; Pardini, Alessandra; Bueno, Ednéia C.
Affiliation
  • Bragazza, Lúcia M; Pontifícia Universidade Católica de Campinas. Faculty of Pharmaceutical Sciences. Campinas. BR
  • Vaz, Adelaide J; University of Säo Paulo. Faculty of Pharmaceutical Sciences. Laboratory of Clinical Immunology. Säo Paulo. BR
  • Passos, Afonso D. C; University of Säo Paulo. Faculty of Medicine. Ribeiräo Preto. BR
  • Takayanagui, Osvaldo M; University of Säo Paulo. Faculty of Medicine. Ribeiräo Preto. BR
  • Nakamura, Paulo M; Adolfo Lutz Institute. Säo Paulo. BR
  • Espíndola, Noeli M; University of Säo Paulo. Faculty of Pharmaceutical Sciences. Laboratory of Clinical Immunology. Säo Paulo. BR
  • Pardini, Alessandra; University of Säo Paulo. Faculty of Pharmaceutical Sciences. Laboratory of Clinical Immunology. Säo Paulo. BR
  • Bueno, Ednéia C; University of Säo Paulo. Faculty of Pharmaceutical Sciences. Laboratory of Clinical Immunology. Säo Paulo. BR
Rev. Inst. Med. Trop. Säo Paulo ; 44(1): 7-12, Jan.-Feb. 2002. ilus, tab, graf
Article in English | LILACS | ID: lil-307235
Responsible library: BR1.1
RESUMO
Considering the impact of cysticercosis on public health, especially the neurologic form of the disease, neurocysticercosis (NC), we studied the frequency of positivity of anti-Taenia solium cysticercus antibodies in serum samples from 1,863 inhabitants of Cássia dos Coqueiros, SP, a municipal district located 80 km from Ribeiräo Preto, an area considered endemic for cysticercosis. The 1,863 samples were tested by enzyme linked immunosorbent assay (ELISA) using an antigenic extract from Taenia crassiceps vesicular fluid (Tcra). The reactive and inconclusive ELISA samples were tested by immunoblotting. Of the 459 samples submitted to immunoblotting, 40 were strongly immunoreactive to the immunodominant 18 and 14 kD peptides. Considering the use of immunoblotting as confirmatory due to its high specificity, the anti-cysticercus serum prevalence in this population was 2.1 percent
Subject(s)
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Full text: Available Collection: International databases Health context: SDG3 - Health and Well-Being / Neglected Diseases Health problem: Target 3.3: End transmission of communicable diseases / Helminthiasis / Neglected Diseases / Zoonoses Database: LILACS Main subject: Taenia / Cysticercosis / Immunoassay / Antibodies, Helminth / Antigens, Helminth Type of study: Diagnostic study / Observational study / Risk factors Limits: Animals / Female / Humans / Male Country/Region as subject: South America / Brazil Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 2002 Document type: Article Affiliation country: Brazil Institution/Affiliation country: Adolfo Lutz Institute/BR / Pontifícia Universidade Católica de Campinas/BR / University of Säo Paulo/BR
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Full text: Available Collection: International databases Health context: SDG3 - Health and Well-Being / Neglected Diseases Health problem: Target 3.3: End transmission of communicable diseases / Helminthiasis / Neglected Diseases / Zoonoses Database: LILACS Main subject: Taenia / Cysticercosis / Immunoassay / Antibodies, Helminth / Antigens, Helminth Type of study: Diagnostic study / Observational study / Risk factors Limits: Animals / Female / Humans / Male Country/Region as subject: South America / Brazil Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 2002 Document type: Article Affiliation country: Brazil Institution/Affiliation country: Adolfo Lutz Institute/BR / Pontifícia Universidade Católica de Campinas/BR / University of Säo Paulo/BR