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Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons
Arganaraz, Enrique R; Hubbard, Gene B; Ramos, Larissa A; Ford, Allen L; Nitz, Nadjar; Leland, Michelle M; Vandeberg, John L; Teixeira, Antonio R. L.
Affiliation
  • Arganaraz, Enrique R; University of Brasília. Faculty of Medicine. Chagas Disease Multidisciplinary Research Laboratory. BR
  • Hubbard, Gene B; Southwest Foundation for Biomedical Research. Department of Laboratory Animal Medicine. San Antonio. US
  • Ramos, Larissa A; University of Brasília. Faculty of Medicine. Chagas Disease Multidisciplinary Research Laboratory. BR
  • Ford, Allen L; Southwest Foundation for Biomedical Research. Department of Physiology and Medicine. San Antonio. US
  • Nitz, Nadjar; University of Brasília. Faculty of Medicine. Chagas Disease Multidisciplinary Research Laboratory. BR
  • Leland, Michelle M; Southwest Foundation for Biomedical Research. Southwest Regional Primate Research Center. Department of Genetics. San Antonio. US
  • Vandeberg, John L; Southwest Foundation for Biomedical Research. Department of Physiology and Medicine. San Antonio. US
  • Teixeira, Antonio R. L; University of Brasília. Faculty of Medicine. Chagas Disease Multidisciplinary Research Laboratory. BR
Rev. Inst. Med. Trop. Säo Paulo ; 43(5): 271-276, Sept.-Oct. 2001. ilus, tab
Article in English | LILACS | ID: lil-308001
Responsible library: BR1.1
ABSTRACT
Trypanosoma cruzi (Schyzotrypanum, Chagas, 1909), and Chagas disease are endemic in captive-reared baboons at the Southwest Foundation for Biomedical Research, San Antonio, Texas. We obtained PCR amplification products from DNA extracted from sucking lice collected from the hair and skin of T. cruzi-infected baboons, with specific nested sets of primers for the protozoan kinetoplast DNA, and nuclear DNA. These products were hybridized to their complementary internal sequences. Selected sequences were cloned and sequencing established the presence of T. cruzi nuclear DNA, and minicircle kDNA. Competitive PCR with a kDNA set of primers determined the quantity of approximately 23.9 18.2 T. cruzi per louse. This finding suggests that the louse may be a vector incidentally contributing to the dissemination of T. cruzi infection in the baboon colony
Subject(s)
Full text: Available Collection: International databases Database: LILACS Main subject: Papio / Lice Infestations / Trypanosoma cruzi / Disease Vectors / Phthiraptera Limits: Animals Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 2001 Document type: Article Affiliation country: Brazil / United States Institution/Affiliation country: Southwest Foundation for Biomedical Research/US / University of Brasília/BR
Full text: Available Collection: International databases Database: LILACS Main subject: Papio / Lice Infestations / Trypanosoma cruzi / Disease Vectors / Phthiraptera Limits: Animals Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 2001 Document type: Article Affiliation country: Brazil / United States Institution/Affiliation country: Southwest Foundation for Biomedical Research/US / University of Brasília/BR
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