Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons
Rev. Inst. Med. Trop. Säo Paulo
; 43(5): 271-276, Sept.-Oct. 2001. ilus, tab
Article
in English
| LILACS
| ID: lil-308001
Responsible library:
BR1.1
ABSTRACT
Trypanosoma cruzi (Schyzotrypanum, Chagas, 1909), and Chagas disease are endemic in captive-reared baboons at the Southwest Foundation for Biomedical Research, San Antonio, Texas. We obtained PCR amplification products from DNA extracted from sucking lice collected from the hair and skin of T. cruzi-infected baboons, with specific nested sets of primers for the protozoan kinetoplast DNA, and nuclear DNA. These products were hybridized to their complementary internal sequences. Selected sequences were cloned and sequencing established the presence of T. cruzi nuclear DNA, and minicircle kDNA. Competitive PCR with a kDNA set of primers determined the quantity of approximately 23.9 18.2 T. cruzi per louse. This finding suggests that the louse may be a vector incidentally contributing to the dissemination of T. cruzi infection in the baboon colony
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Papio
/
Lice Infestations
/
Trypanosoma cruzi
/
Disease Vectors
/
Phthiraptera
Limits:
Animals
Language:
English
Journal:
Rev. Inst. Med. Trop. Säo Paulo
Journal subject:
Tropical Medicine
Year:
2001
Document type:
Article
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
Southwest Foundation for Biomedical Research/US
/
University of Brasília/BR