Comparison of electrophoretic protein profiles of campylobacter jejuni subsp. jejuni isolated from different animal species
Braz. j. microbiol
; 32(4): 286-292, Oct.-Dec. 2001. ilus, tab, graf
Article
in English
| LILACS
| ID: lil-314799
Responsible library:
BR32.1
RESUMO
Electrophoretic protein profiles of Campylobacter jejuni subsp. jejuni strains isolated from feces of seven animal species, including man, were compared. Fourteen strains (two from each species) plus two human strains and the reference one, were ruptured by ultrasound and their total soluble proteins were analyzed by SDS-PAGE technique in a 12(per cent) polyacrylamide gel with computerized densitometric reading by the molecular analyst software. All the strains had bands in common that correspond to 45 and 66 Kda molecular weight. The disagreement corresponded to a 97 to 200 Kda molecular weight region. From the 17 strains, 13 (76.5 per cent), were classified as biotype I, three (17.6 per cent) as biotype II and one (5.8 per cent) as biotype III. Since protein extracts were obtained from cells grown under identical conditions, and thus, able to express the same phenotype, this disagreement region could be related to different genotypes or serotypes.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Bacterial Proteins
/
In Vitro Techniques
/
Campylobacter jejuni
/
Bacterial Typing Techniques
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2001
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Biológico/BR
/
Universidade de São Paulo/BR