Dried blood spots collected on filter paper: an international resource for the diagnosis and genetic characterization of human immunodeficiency virus type-1
Mem. Inst. Oswaldo Cruz
; 91(3): 351-358, May-Jun. 1996.
Article
in En
| LILACS
| ID: lil-319863
Responsible library:
BR1.1
RESUMO
The collection of dried blood spots (DBS) on filter paper provides a powerful approach for the development of large-scale, population-based screening programs. DBS methods are particularly valuable in developing countries and isolated rural regions where resources are limited. Large numbers of field specimens can be economically collected and shipped to centralized reference laboratories for genetic and (or) serological analysis. Alternatively, the dried blood can be stored and used as an archival resource to rapidly establish the frequency and distribution of newly recognized mutations, confirm patient identity or track the origins and emergence of newly identified pathogens. In this report, we describe how PCR-based technologies are beginning to interface with international screening programmes for the diagnosis and genetic characterization of human immunodeficiency virus type 1 (HIV-1). In particular, we review recent progress using DBS specimens to resolve the HIV-1 infection status of neonates, monitor the genetic evolution of HIV-1 during early infancy and establish a sentinel surveillance system for the systematic monitoring of HIV-1 genetic variation in Asia.
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Paper
/
AIDS Serodiagnosis
/
Blood Specimen Collection
/
Acquired Immunodeficiency Syndrome
/
HIV-1
/
Filtration
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
/
Infant
/
Newborn
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
1996
Document type:
Article
Affiliation country:
Bahamas
/
Canada
/
India
/
United States
Country of publication:
Brazil