Isolation of a distally located gene possibly correlated with gametocyte production ability
Mem. Inst. Oswaldo Cruz
; 89(Suppl.2): 33-35, 1994.
Article
in English
| LILACS
| ID: lil-319955
Responsible library:
BR1.1
RESUMO
Previous studies were focussed on the attempt to correlate observable variations in the size of Plasmodium berghei chromosomes with the loss of ability to produce viable gametocytes. A temporal coincidence between the appearance of a subtelomeric deletion on P. berghei chromosome 5 and the loss of the ability to produce viable gametocytes was observed in a clone (HPE) directly derived from the high gametocyte-producer clone 8417 during mechanical passages. Interestingly enough, three P. berghei sexual-specific genes have already been mapped on internal fragments of this chromosome. A novel gene, clone 150, isolated from a genomic library of clone 8417 using a probe enriched for sexual-specific transcripts, maps on chromosome 5 within 100kb from the telomere. Subtelomeric deletions of chromosome 5 affecting two non-producer clones involve part of the transcribed region of this gene.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Plasmodium berghei
/
Chromosome Mapping
/
Genes, Protozoan
/
Gametogenesis
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1994
Document type:
Article
Affiliation country:
Italy
Institution/Affiliation country:
Istituto Superiore di Sanità/IT