Nuclear phenotype changes after heat shock in Panstrongylus megistus (Burmeister)
Mem. Inst. Oswaldo Cruz
; 95(2): 271-277, Mar.-Apr. 2000.
Article
in English
| LILACS, Sec. Est. Saúde SP
| ID: lil-319964
Responsible library:
BR1.1
RESUMO
The nuclear phenotypes of Malpighian tubule epithelial cells of male nymphs of the blood-sucking insect, Panstrongylus megistus, subjected to short- and long-duration heat shocks at 40oC were analyzed immediately after the shock and 10 and 30 days later. Normal nuclei with a usual heterochromatic body as well as phenotypes indicative of survival (unravelled heterochromatin, giants) and death (apoptosis, necrosis) responses were observed in control and treated specimens. However, all nuclear phenotypes, except the normal ones, were more frequent in shocked specimens. Similarly altered phenotypes have also been reported in Triatoma infestans following heat shock, although at different frequencies. The frequency of the various nuclear phenotypes observed in this study suggests that the forms of cell survival observed were not sufficient or efficient enough to protect all of the Malpighian tubule cells from the deleterious effects of stress. In agreement with studies on P. megistus survival following heat shock, only long-duration shock produced strongly deleterious effects.
Full text:
Available
Collection:
National databases
/
Brazil
Database:
LILACS
/
Sec. Est. Saúde SP
Main subject:
Panstrongylus
/
Cell Nucleus
/
Hot Temperature
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Year:
2000
Document type:
Article
Institution/Affiliation country:
SUCEN/BR
/
Universidade Estadual de Campinas/BR