Cross-reactivity of antibodies in human infections by the kinetoplastid protozoa Trypanosoma cruzi, Leishmania chagasi and Leishmania (viannia) braziliensis
Rev. Inst. Med. Trop. Säo Paulo
; 38(3): 177-185, May-Jun. 1996.
Article
in English
| LILACS, Sec. Est. Saúde SP
| ID: lil-320648
Responsible library:
BR1.1
ABSTRACT
We have detected antibodies, in the sera of Chagas disease, Kala-azar and Mucocutaneous leishmaniasis patients, that bind multiple antigens shared between the three causative agents. The Chagas disease sera showed 98 to 100 positive results by ELISA when the Leishmania braziliensis and Leishmania chagasi antigens were used, respectively. The Kala-azar sera showed 100 positive results with Trypanosoma cruzi or L. braziliensis antigens by immunofluorescence assays. The antibodies in the sera of Mucocutaneous leishmaniasis patients showed 100 positive results by ELISA assays with T. cruzi or L. chagasi antigens. Furthermore, the direct agglutination of L. chagasi promastigotes showed that 95 of Kala-azar and 35 of Mucocutaneous leishmaniasis sera agglutinated the parasite in dilutions above 1512. In contrast, 15 of Chagas sera agglutinated the parasite in dilutions 116 and below. Western blot analysis showed that the Chagas sera that formed at least 24 bands with the T. cruzi also formed 13 bands with the L. chagasi and 17 bands with the L. braziliensis. The Kala-azar sera that recognized at least 29 bands with the homologous antigen also formed 14 bands with the T. cruzi and 10 bands with the L. braziliensis antigens. Finally, the Mucocutaneous leishmaniasis sera that formed at least 17 bands with the homologous antigen also formed 10 bands with the T. cruzi and four bands with the L. chagasi antigens. These results indicate the presence of common antigenic determinants in several protozoal proteins and, therefore, explain the serologic cross-reactions reported here.
Full text:
Available
Collection:
National databases
/
Brazil
Health context:
Neglected Diseases
Health problem:
Chagas Disease
/
Neglected Diseases
/
Zoonoses
Database:
LILACS
/
Sec. Est. Saúde SP
Main subject:
Antibodies, Protozoan
/
Leishmaniasis, Mucocutaneous
/
Chagas Disease
/
Leishmaniasis, Visceral
Limits:
Animals
/
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Rev. Inst. Med. Trop. Säo Paulo
Year:
1996
Document type:
Article
Institution/Affiliation country:
Universidade de Brasilia/BR