Your browser doesn't support javascript.
loading
Genetic instability of sugarcane plants derived from meristem cultures
Zucchi, Maria Imaculada; Arizono, Hideto; Morais, Vicente Alberto; Fungaro, Maria Helena Pelegrinelli; Vieira, Maria Lúcia Carneiro.
Affiliation
  • Zucchi, Maria Imaculada; Escola Superior de Agricultura "Luiz de Queiroz". Departamento de Genética. Piracicaba. BR
  • Arizono, Hideto; Universidade Federal de Säo Carlos. Centro de Ciências Agrárias. Departamento de Biotecnologia Vegetal. Araras. BR
  • Morais, Vicente Alberto; Centro de Tecnologia Copersucar. Seçäo de Fitopatologia. Piracicaba. BR
  • Fungaro, Maria Helena Pelegrinelli; Universidade Estadual de Londrina. Departamento de Biologia Geral. Londrina. BR
  • Vieira, Maria Lúcia Carneiro; Escola Superior de Agricultura "Luiz de Queiroz". Departamento de Genética. Piracicaba. BR
Genet. mol. biol ; 25(1): 91-96, 2002. ilus, tab
Article in English | LILACS | ID: lil-324993
Responsible library: BR26.1
ABSTRACT
The RADP (Random amplified polymorphic DNA) technique was used to detect tissue-culture-induced variations in sugarcane. Plants of the Brazilian variety RB83-5486 propagated via rhizomes and via meristem cultures were studied. The polymorphism rate for 98 RAPD loci was 6.93 percent when the plants derived from meristems. Besides, in order to evaluate the influence of the number of subcultures on the generation of somaclonal variation, field-grown RB83-5486 plants derived from 10 meristems were studied after five subcultivations. Although different rates of polymorphism were observed, there was no direct association with the stage of subcultivation. The analysis of plants of two sugarcane varieties cultivated in vitro from meristems showed that variety RB83-5486 was more unstable than variety SP80-185
Subject(s)
Fulltext
Add to My VHL
Print
XML
Search on Google
Full text: Available Collection: International databases Database: LILACS Main subject: Plants / Genetic Variation / Nucleic Acid Amplification Techniques Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2002 Document type: Article Affiliation country: Brazil Institution/Affiliation country: Centro de Tecnologia Copersucar/BR / Escola Superior de Agricultura "Luiz de Queiroz"/BR / Universidade Estadual de Londrina/BR / Universidade Federal de Säo Carlos/BR
Fulltext
Add to My VHL
Print
XML
Search on Google
Full text: Available Collection: International databases Database: LILACS Main subject: Plants / Genetic Variation / Nucleic Acid Amplification Techniques Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2002 Document type: Article Affiliation country: Brazil Institution/Affiliation country: Centro de Tecnologia Copersucar/BR / Escola Superior de Agricultura "Luiz de Queiroz"/BR / Universidade Estadual de Londrina/BR / Universidade Federal de Säo Carlos/BR