Different culture media applied to the study of Cryptococcus neoformans susceptibility to amphotericin B and fluconazole
Braz. j. microbiol
; 33(1): 27-30, jan.-mar. 2002. tab
Article
in English, Portuguese
| LILACS
| ID: lil-325363
Responsible library:
BR32.1
RESUMO
This study compared the performance of three culture media used for the determination of minimal inhibitory concentration (MIC) of amphotericin B and fluconazole against 48 Cryptococcus neoformans strains. For amphotericin B the media employed were RPMI 1640 medium, as indicated by NCCLS as reference, Yeast Nitrogen Base (YNB) as proposed by Ghannoum et al. (YNB-1), Antibiotic medium 3 (AM3) indicated by others, and YNB as proposed by us (YNB-2). In YNB-1, amphotericin B showed broader MIC ranges, varying from 0.125 µg/ml to 1.0 µg/ml; in RPMI 1640 medium and YNB-2 the MICs were similar and in AM3 the MIC ranges were narrower. The same pattern was observed for MIC 90 (per cent). For fluconazole the media employed were RPMI 1640 and YNB-1. The MIC ranges were broader and elevated when YNB-1 was employed. MIC 50 (per cent) were 2.0 µg/ml in RPMI 1640 medium and 4.0 µg/ml in YNB-1. Our results indicate that RPMI 1640 supplemented with (2 per cent) glucose allowed a good growth at 48h; YNB as recommended by Ghannoum et al. provided a broader MIC range, AM3 provided a narrow MIC range for amphotericin B, and YNB-2 was very similar to RPMI 1640 medium.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
In Vitro Techniques
/
Cryptococcus neoformans
/
Antifungal Agents
Language:
English
/
Portuguese
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2002
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Hospital Universitário de Santa Maria/BR
/
Universidade Federal de Santa Maria Santa Maria, RS, Brasil/BR
/
Universidade Federal de Santa Maria/BR