Addition of exogenous cytokines in mixed lymphocyte culture for selecting related donors for bone marrow transplantation
São Paulo med. j
; 120(6): 175-179, 2002. graf
Article
in English
| LILACS
| ID: lil-326357
Responsible library:
BR1.1
RESUMO
CONTEXT Mixed lymphocyte culturing has led to conflicting opinions regarding the selection of donors for bone marrow transplantation. The association between a positive mixed lymphocyte culture and the development of graft-versus-host disease (GVHD) is unclear. The use of exogenous cytokines in mixed lymphocyte cultures could be an alternative for increasing the sensitivity of culture tests. OBJECTIVE:
To increase the sensitivity of mixed lymphocyte cultures between donor and recipient human leukocyte antigen (HLA) identical siblings, using exogenous cytokines, in order to predict post-transplantation GVHD and/or rejection. TYPE OF STUDY Prospective study.SETTING:
Bone Marrow Transplantation Unit, Universidade Estadual de Campinas.PARTICIPANTS:
Seventeen patients with hematological malignancies and their respective donors selected for bone marrow transplantation procedures. PROCEDURES Standard and modified mixed lymphocyte culturing by cytokine supplementation was carried out using donor and recipient cells typed for HLA. MAIN MEASUREMENTS Autologous and allogenic responses in mixed lymphocyte cultures after the addition of IL-4 or IL-2.RESULTS:
In comparison with the standard method, average responses in the modified mixed lymphocyte cultures increased by a factor of 2.0 using IL-4 (p < 0.001) and 6.4 using IL-2 (p < 0.001), for autologous donor culture responses. For donor-versus-recipient culture responses, the increase was by a factor of 1.9 using IL-4 (p < 0.001) and 4.1 using IL-2 (p < 0.001). For donor-versus-unrelated culture responses, no significant increase was observed using IL-4, and a mean response inhibition of 20 percent was observed using IL-2 (p < 0.001). Neither of the cytokines produced a significant difference in the unrelated control versus recipient cell responses.CONCLUSION:
IL-4 supplementation was the best for increasing the mixed lymphocyte culture sensitivity. However, IL-4 also increased autologous responses, albeit less intensively than IL-2. Thus, with this loss of specificity we believe that it is not worth modifying the traditional mixed lymphocyte culture method, even with IL-4 addition
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Tissue Donors
/
Cytokines
/
Bone Marrow Transplantation
/
Graft Rejection
/
Graft vs Host Disease
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
/
Risk factors
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
English
Journal:
São Paulo med. j
Journal subject:
Cirurgia Geral
/
Cincia
/
Ginecologia
/
Medicine
/
Medicina Interna
/
Obstetr¡cia
/
Pediatria
/
Sa£de Mental
/
Sa£de P£blica
Year:
2002
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual de Campinas/BR
/
Universidade Estadual de Maringá/BR