Falsos cultivos positivos por contaminación cruzada en laboratorios de tuberculosis / False-positive cultures due to cross contamination in tuberculosis laboratories
Rev. argent. microbiol
; 34(3): 163-166, jul.-sept. 2002.
Article
in Spanish
| LILACS
| ID: lil-331788
Responsible library:
BR1.1
ABSTRACT
Fifteen episodes of Mycobacterium tuberculosis laboratory cross-contamination suspected between 1996 and 2001 at 6 laboratories in Buenos Aires City and suburbs were investigated by IS6110 RFLP. Thirteen episodes were confirmed. Even though BACTEC 460 produced the highest number of confirmed episodes in a single laboratory, the most extended one occurred while employing conventional culture procedures in solid medium. The double repetitive element-polymerase chain reaction (DRE-PCR) was applied to 8 of these episodes and produced concordant results with those of the RFLP. The DRE-PCR appears to be a valuable tool for the prompt identification of false positive cultures. The timely rectification of defects in laboratory protocols can avert false diagnoses of tuberculosis and unnecessary prolonged treatments.
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Collection:
International databases
Health context:
SDG3 - Health and Well-Being
/
Neglected Diseases
/
SDG3 - Target 3.3 End transmission of communicable diseases
Health problem:
Target 3.3: End transmission of communicable diseases
/
Neglected Diseases
/
Tuberculosis
/
Tuberculosis
Database:
LILACS
Main subject:
Tuberculosis
/
Polymorphism, Restriction Fragment Length
/
DNA, Bacterial
/
Laboratories, Hospital
/
Polymerase Chain Reaction
/
Equipment Contamination
/
Bacteriological Techniques
/
Mycobacterium tuberculosis
Type of study:
Practice guideline
Limits:
Humans
Country/Region as subject:
South America
/
Argentina
Language:
Spanish
Journal:
Rev. argent. microbiol
Journal subject:
Microbiology
Year:
2002
Document type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Hospital F. J. Muñiz/AR
/
Hospital Interzonal General de Agudos Evita de Lanús/AR
/
Hospital J. Fernández/AR
/
Hospital J. Garrahan/AR
/
Hospital Municipal de San Isidro/AR
/
INEI ANLIS Carlos G. Malbrán/AR