Infección in vitro por diferentes cepas de Paracoccidioides brasiliensis / In vitro infection by different strains of Paracoccidioides brasiliensis
Rev. argent. microbiol
; 32(3): 116-122, jul.-sept. 2000.
Article
in Spanish
| LILACS
| ID: lil-332527
Responsible library:
BR1.1
RESUMO
We analyzed the in vitro infection process by P. brasiliensis and the effect of extracellular factor(s) produced on monolayers of mammalian Vero cell lines. The yeast phase of four strains was studied B339 (avirulent or slightly virulent), U, (intermediate virulence), 93745 and 63265 (both highly virulent). Strains of intermediate and high virulence had higher adherence at first contact (about 16). Strain B339 had a slower adherence at first contact (8) than the others during the same period. The production of extracellular proteases, soluble extracellular factor(s) and extracellular antigen gP43 showed no correlation with the in vitro physiopathogenicity of the analyzed strains. We demonstrate that the Vero model presented in this paper is a suitable system to study infection and virulence in vitro. We are currently assessing its usefulness as a tool for the analysis of the interaction between pathogen, host and antifungal agents.
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Collection:
International databases
Database:
LILACS
Main subject:
Paracoccidioides
/
Mycology
Type of study:
Prognostic study
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
Spanish
Journal:
Rev. argent. microbiol
Journal subject:
Microbiology
Year:
2000
Document type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Instituto Nacional de Enfermedades Infecciosas ANLIS Dr. Carios G. Malbrán/AR