Transient gene expression of beta-glucuronidase in citrus thin epicotyl transversal sections using particle bombardment
Braz. arch. biol. technol
; 46(1): 1-6, Jan. 2003. ilus, graf
Article
in English
| LILACS
| ID: lil-334442
Responsible library:
BR1.1
ABSTRACT
Studies were carried out to optimize the conditions for transient gene expression through particle bombardment on Carrizo citrange (Citrus sinensis x Poncirus trifoliata) thin epicotyl sections. The best conditions for transient GUS expression were M-25 tungsten particles, 1550 psi helium pressure, 9 cm distance between specimen and DNA/particle holder and culture of explants in a high osmolarity medium (0.2 M mannitol + 0.2 M sorbitol) 4 h prior and 20 h after bombardment. Under these conditions, an average of 102 blue spots per bombardment (20 explants/plate) were achieved. This protocol is currently being used for transformation of Carrizo citrange and sweet orange (Citrus sinensis)
Full text:
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Collection:
International databases
Database:
LILACS
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2003
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Agronômico do Paraná/BR