Preservation of goat preantral follicles in saline or coconut water solution
Braz. j. vet. res. anim. sci
; 39(6): 324-330, 2002. ilus, graf
Article
in English
| LILACS
| ID: lil-337385
Responsible library:
BR68.1
RESUMO
The present study investigated the efficiency of saline solution and coconut water solution in the preservation of goat preantral follicles enclosed in ovarian tissue, at different temperatures and for different incubation periods. At the slaughterhouse, the ovarian pair was divided into 19 fragments; one ovarian fragment was immediately fixed for histology (control-time zero). The other 18 ovarian fragments were preserved in both solutions at 4ºC, 20ºC or 39ºC for 4 h, 12 h or 24 h. The histological analysis showed that the storage of ovarian fragments in both solutions at 4ºC for up to 24 h kept the percentage of normal preantral follicles similar to the control values. In contrast, preservation at 20°C or 39ºC, in either solution, reduced significantly the percentage of normal preantral follicles compared to the control values, except in saline solution at 20ºC for 4 h or in coconut water solution at 20ºC for 4 h and 12 h. In conclusion, this study shows that both solutions can be used with the same efficiency to preserve goat preantral follicles at 4°C, irrespective of the incubation time. However, to preserve goat preantral follicles at higher temperatures, coconut water solution is recommended
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Goats
/
Ovarian Follicle
Language:
English
Journal:
Braz. j. vet. res. anim. sci
Journal subject:
Veterinary Medicine
Year:
2002
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual do Ceará/BR
/
Universidade Federal do Pará/BR