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Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons
Mafra, R. A; Leäo, R. M; Beiräo, P. S. L; Cruz, J. S.
Affiliation
  • Mafra, R. A; Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Membranas Excitáveis. Belo Horizonte. BR
  • Leäo, R. M; Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Membranas Excitáveis. Belo Horizonte. BR
  • Beiräo, P. S. L; Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Membranas Excitáveis. Belo Horizonte. BR
  • Cruz, J. S; Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Membranas Excitáveis. Belo Horizonte. BR
Braz. j. med. biol. res ; 36(7): 951-957, July 2003. ilus, graf
Article in English | LILACS | ID: lil-340691
Responsible library: BR1.1
RESUMO
A glutamate-sensitive inward current (Iglu) is described in rat cerebellar granule neurons and related to a glutamate transport mechanism. We examined the features of Iglu using the patch-clamp technique. In steady-state conditions the Iglu measured 8.14 ± 1.9 pA. Iglu was identified as a voltage-dependent inward current showing a strong rectification at positive potentials. L-Glutamate activated the inward current in a dose-dependent manner, with a half-maximal effect at about 18 æM and a maximum increase of 51.2 ± 4.4 percent. The inward current was blocked by the presence of dihydrokainate (0.5 mM), shown by others to readily block the GLT1 isoform. We thus speculate that Iglu could be attributed to the presence of a native glutamate transporter in cerebellar granule neurons
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Full text: Available Collection: International databases Database: LILACS Main subject: Cerebellum / Neuroglia Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2003 Document type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Minas Gerais/BR
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Full text: Available Collection: International databases Database: LILACS Main subject: Cerebellum / Neuroglia Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2003 Document type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Minas Gerais/BR