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Detection of rotavirus in dogs with diarrhea in Brazil
Gabbay, Yvone B; Homem, Valéria S. F; Munford, Veridiana; Alves, Antonia S; Mascarenhas, Joana D. P; Linhares, Alexandre C; Rácz, Maria Lúcia.
Affiliation
  • Gabbay, Yvone B; Fundação Nacional de Saúde. Instituto Evandro Chagas. Seção de Virologia Geral. BR
  • Homem, Valéria S. F; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal. São Paulo. BR
  • Munford, Veridiana; Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Microbiologia. São Paulo. BR
  • Alves, Antonia S; Fundação Nacional de Saúde. Instituto Evandro Chagas. Seção de Virologia Geral. BR
  • Mascarenhas, Joana D. P; Fundação Nacional de Saúde. Instituto Evandro Chagas. Seção de Virologia Geral. BR
  • Linhares, Alexandre C; Fundação Nacional de Saúde. Instituto Evandro Chagas. Seção de Virologia Geral. BR
  • Rácz, Maria Lúcia; Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Microbiologia. São Paulo. BR
Braz. j. microbiol ; 34(1): 77-80, Jan.-Apr. 2003. ilus
Article in English | LILACS | ID: lil-344570
Responsible library: BR32.1
RESUMO
Rotavirus was detected by the enzyme-linked immunosorbent assay (ELISA) in the faeces of a diarrheic dog. Virus particles with morphology typical of rotavirus were visualized by direct electron microscopy. This sample was subsequently tested for the four main human serotypes (G1-G4), by ELISA with monoclonal antibodies. G genotyping was attempted by RT-PCR using G1-G6 and G8-G11 primers but no positive results could be yielded. Also using RT-PCR it was possible to characterize this canine strain as belonging to P[ 3] genotype. This is the first canine rotavirus detected in Brazil.
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Full text: Available Collection: International databases Health context: Neglected Diseases Health problem: Diarrhea / Neglected Diseases Database: LILACS Main subject: In Vitro Techniques / Enzyme-Linked Immunosorbent Assay / Rotavirus / Cuspid / Diarrhea Type of study: Diagnostic study Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2003 Document type: Article Affiliation country: Brazil Institution/Affiliation country: Fundação Nacional de Saúde/BR / Universidade de São Paulo/BR
Fulltext
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Full text: Available Collection: International databases Health context: Neglected Diseases Health problem: Diarrhea / Neglected Diseases Database: LILACS Main subject: In Vitro Techniques / Enzyme-Linked Immunosorbent Assay / Rotavirus / Cuspid / Diarrhea Type of study: Diagnostic study Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2003 Document type: Article Affiliation country: Brazil Institution/Affiliation country: Fundação Nacional de Saúde/BR / Universidade de São Paulo/BR