Aplicación del tiempo de tromboplastina parcial activado (TTPA) con tiempo de incubación modificado en el diagnóstico del anticoagulante lúpico (AL) / Clinical application of the activated partial thromboplastin time (APTT) with modified incubation time for the diagnosis of lupus anticoagulant (LA)
Rev. méd. Chile
; 132(4): 407-412, abr. 2004. tab
Article
in Spanish
| LILACS
| ID: lil-362904
Responsible library:
CL12.1
RESUMO
Background:
Although several tests are used to screen for the presence of LA, none detects all its types. The shortening of APTT observed when the pre-incubation period is prolonged, proved to be a sensitive test for the presence of LA. Material andmethods:
We determined the APTT, performed with a 4 or 15 min preincubation period (APTTs and APTT15 respectively), in 22 healthy subjects, 3 commercial positive controls for LA, 16 patients with a previous diagnosis of LA and 54 patients with recurrent fetal loss and/or infertility. Evidence of LA was established by a positive Staclot-LA test.Results:
APTTs and APTT15 were 31.5±4.7 and 28.4±4.5 seconds respectively in samples from 22 normal subjects. The figures in samples with LA, were 71.5±20.3 s and 58.6±18 s respectively. The difference between the two APTTs performed on an individual sample was defined as the APTT 4-15 and was 2.6±2.0 in normal subjects 2.5±2.8 in 13 patients anticoagulated with warfarin, -10.0±6.5 in 13 patients receiving heparin, and 13.2±4.9 in 15 patients with LA. The test values for LA patients were significantly higher than those for normal subjects (p <0.0001). For values over 5, the APTT 4-15 had 93.3 per cent sensitivity and 100 per cent specificity. In one patient with recurrent fetal loss or infertility, who was LA positive, the APTT 4-15 was positive with a value of 14.Conclusions:
This modified TTPA is easy to perform, and provides a reasonably discriminatory value for the presence of LA. Therefore, we recommend the TTPA 4-15 to screen for LA.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Partial Thromboplastin Time
/
Lupus Coagulation Inhibitor
/
Lupus Erythematosus, Systemic
Type of study:
Diagnostic study
Limits:
Female
/
Humans
/
Pregnancy
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2004
Document type:
Article
Affiliation country:
Chile
/
United States
Institution/Affiliation country:
Universidad de Alabama en Birmingham/US
/
Universidad de Chile/CL