Typing class I HLA-A gene using a nested PCR-RFLP procedure
Braz. j. med. biol. res
; 38(6): 837-842, June 2005. ilus, tab
Article
in English
| LILACS
| ID: lil-402664
Responsible library:
BR1.1
ABSTRACT
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95 percent. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Histocompatibility Testing
/
Genes, MHC Class I
/
HLA-A Antigens
/
Reverse Transcriptase Polymerase Chain Reaction
Limits:
Humans
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2005
Document type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual Paulista/BR
/
Universidade de São Paulo/BR