Identification and complete sequencing of novel human transcripts through the use of mouse orthologs and testis cDNA sequences
Genet. mol. res. (Online)
; 3(4): 493-511, 2004. tab, graf
Article
in English
| LILACS
| ID: lil-410894
Responsible library:
BR1.1
ABSTRACT
The correct identification of all human genes, and their derived transcripts, has not yet been achieved, and it remains one of the major aims of the worldwide genomics community. Computational programs suggest the existence of 30,000 to 40,000 human genes. However, definitive gene identification can only be achieved by experimental approaches. We used two distinct methodologies, one based on the alignment of mouse orthologous sequences to the human genome, and another based on the construction of a high-quality human testis cDNA library, in an attempt to identify new human transcripts within the human genome sequence. We generated 47 complete human transcript sequences, comprising 27 unannotated and 20 annotated sequences. Eight of these transcripts are variants of previously known genes. These transcripts were characterized according to size, number of exons, and chromosomal localization, and a search for protein domains was undertaken based on their putative open reading frames. In silico expression analysis suggests that some of these transcripts are expressed at low levels and in a restricted set of tissues.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Testis
/
Transcription, Genetic
/
Genome, Human
/
Sequence Analysis, DNA
/
DNA, Complementary
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Genet. mol. res. (Online)
Journal subject:
Molecular Biology
/
Genetics
Year:
2004
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Ludwig de Pesquisa sobre o Câncer/BR
/
Faculdade de Medicina de São José do Rio Preto/BR
/
Ludwig Institute for Cancer Research/BR
/
Universidade Estadual Paulista/BR
/
Universidade Estadual de Campinas/BR
/
Universidade Federal de São Carlos/BR
/
Universidade Federal de São Paulo/BR
/
Universidade de Ribeirão Preto/BR
/
Universidade de São Paulo/BR