Quantification of 4-nerolidylcatechol from Pothomorphe umbellata (Piperaceae) in rat plasma samples by HPLC-UV
RBCF, Rev. bras. ciênc. farm. (Impr.)
; 40(3): 373-380, jul.-set. 2004. ilus, tab, graf
Article
in English
| LILACS
| ID: lil-420685
Responsible library:
BR40.1
RESUMO
The oral dosages formulations from Pothomorphe umbellata (Piperaceae) can only be therapeutically evaluated after establishing 4-nerolidylcatechol (4-NRC) pharmacokinetic profile in plasma. For that purpose, a unique analytical method validation using HPLC-UV detection was developed for analysis of rat plasma samples. The animals received 10 mg/kg b.w. of 4-NRC i.v.. Analytical conditions were set with C18 column, methanol acetonitrile water (622018) as mobile phase, and a flow rate of 1.6 mL/min. The assay was linear from 1.0 - 80.0 mcg/mL. The recovery procedure was performed by liquid-liquid extraction showing quantitative extraction (106.4 ± 8.7 por cento)...
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Plants, Medicinal
/
Technology, Pharmaceutical
/
Herbal Medicine
Limits:
Animals
Language:
English
Journal:
RBCF, Rev. bras. ciênc. farm. (Impr.)
Journal subject:
Biochemistry
/
Pharmacy
/
Pharmacology
Year:
2004
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade de São Paulo/BR