Determination of fluconazole in serum and amniotic fluid of rats by gas-chromatography/mass spectrometry (GC/MS)
RBCF, Rev. bras. ciênc. farm. (Impr.)
; 41(2): 225-227, abr.-jun. 2005. tab, graf
Article
in English
| LILACS
| ID: lil-420703
Responsible library:
BR40.1
RESUMO
Rats treated with oral dose of 100 mg/kg of fluconazole during pregnancy had their serum and amniotic fluid quantified for this drug using a GC/MS method. Fluconazole was extracted with ethyl acetate from samples and analysed by a GC-MS Shimadzu QP5050A system using a CBP-5 fused silica capillary column. Tioconazole was used as internal standard. Calibration curve was linear within the range 10.0 - 300.0 µg/mL. The limit of quantification was 0.1 µg/mL and no interference was observed in the blank serum and amniotic liquid. The mean concentrations of the drug in the serum and amniotic fluid were 206.01 ± 105.25 µg/mL and 125.34 ± µg/mL and 125.34 ± 65.24 µg/mL, respectively. This procedure showed to be sensitive and efficient enough for the use in teratogenic studies of fluconazole and other azole drugs
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Fluconazole
/
Amniotic Fluid
Limits:
Animals
Language:
English
Journal:
RBCF, Rev. bras. ciênc. farm. (Impr.)
Journal subject:
Biochemistry
/
Pharmacy
/
Pharmacology
Year:
2005
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Goiás/BR