Sequence characterization of coding regions of the myostatin gene ( GDF8 ) from Brazilian Murrah buffaloes ( Bubalus bubalis ) and comparison with the Bos taurus sequence
Genet. mol. biol
; 29(1): 79-82, 2006. ilus, tab
Article
in English
| LILACS
| ID: lil-424740
Responsible library:
BR26.1
RESUMO
Within about 30 years the Brazilian buffalo (Bubalus bubalis) herd will reach approximately 50 million head as a result of the great adaptive capacity of these animals to tropical climates, together with the good productive and reproductive potential which make these animals an important animal protein source for poor and developing countries. The myostatin gene (GDF8) is important in the physiology of stock animals because its product produces a direct effect on muscle development and consequently also on meat production. The myostatin sequence is known in several mammalian species and shows a high degree of amino acid sequence conservation, although the presence of non-silent and silent changes in the coding sequences and several alterations in the introns and untranslated regions have been identified. The objective of our work was to characterize the myostatin coding regions of B. bubalis (Murrah breed) and to compare them with the Bos taurus regions looking for variations in nucleotide and protein sequences. In this way, we were able to identify 12 variations at DNA level and five alterations on the presumed myostatin protein sequence as compared to non double-muscled bovine sequences.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Buffaloes
/
Myogenic Regulatory Factors
Type of study:
Prognostic study
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2006
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual Paulista Júlio de Mesquita Filho/BR
/
Universidade Federal do Pará/BR