Rapid, reliable and inexpensive quality assessment of biotinylated cRNA
Braz. j. med. biol. res
; 39(5): 589-593, May 2006. ilus, tab
Article
in English
| LILACS
| ID: lil-425790
Responsible library:
BR1.1
RESUMO
The interpretation of oligonucleotide array experiments depends on the quality of the target cRNA used. cRNA target quality is assessed by quantitative analysis of the representation of 5' and 3' sequences of control genes using commercially available Test arrays. The Test array provides an economically priced means of determining the quality of labeled target prior to analysis on whole genome expression arrays. This manuscript validates the use of a duplex RT-PCR assay as a faster (6 h) and less expensive (
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
RNA, Complementary
/
Reverse Transcriptase Polymerase Chain Reaction
/
Oligonucleotide Array Sequence Analysis
Limits:
Humans
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2006
Document type:
Article
/
Project document
Affiliation country:
United States
Institution/Affiliation country:
Harvard Medical School/US