Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection
Rev. Soc. Bras. Med. Trop
; 39(2): 159-162, mar.-abr. 2006. ilus, tab
Article
in English
| LILACS, Sec. Est. Saúde SP
| ID: lil-426908
Responsible library:
BR1.1
ABSTRACT
A digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100 percent agreement. The hybridization, when carried out in products amplified by RT-PCR, increases the sensitivity of this technique even more and confers specificity to the diagnosis. The technique described in this work will be useful in rabies diagnosis laboratories, once the cost is compatible with traditional rabies diagnostic techniques.
Full text:
Available
Collection:
National databases
/
Brazil
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
LILACS
/
Sec. Est. Saúde SP
Main subject:
Rabies
/
Rabies virus
/
DNA, Viral
/
Digoxigenin
/
Nucleoproteins
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Year:
2006
Document type:
Article
Institution/Affiliation country:
Institute Pasteur São Paulo/BR
/
University of São Paulo/BR