Estudio del patrón de metilación génico en el cáncer gástrico en Chile / Promoter methylation profile in gastric cancer
Rev. méd. Chile
; 133(8): 874-880, ago. 2005. ilus, tab
Article
in Spanish
| LILACS
| ID: lil-429220
Responsible library:
BR1.1
RESUMEN
Background:
Promoter genomic DNA methylation is an important inactivation mechanism of tumor suppressor genes. This genetic-molecular pathway for cancer may separate a subset of patients with different prognoses and eventually different responses to specific therapies.Aim:
To analyze the methylation pattern of important genes related to different carcinogenic mechanisms in patients with gastric cancer (GC) and the relationship with its morphological features and biological behavior. Material andmethods:
Forty-seven fresh-frozen GC samples were selected. The methylation-specific PCR (MSP) test was used to analyze promoter methylation status for genes MLH1, CDKN2A (p16), APC, CDH1 (Cadherin E) and FHIT. Follow-up and complete morphological features were obtained for all cases.Results:
We found methylation in at least one of the genes studied in 83% of the cases. The frequencies of promoter hypermethylation of MLH1, CDKN2A, APC, CDH1 and FHIT were 31%, 43%, 46%, 80% y 62%, respectively. We found a relationship between APC methylation and good histological differentiation (p=0.03); CDH1 methylation with diffuse type by Lauren and 3 or more metastasic lymph nodes (p <0.05); FHIT, CDKN2A and CDH1 methylation and female condition (p <0.04). We also found a non-significant relationship between CDKN2A methylation and better survival (p=0.07).Conclusions:
The high frequency promoter methylation found confirms its importance in gastric carcinogenesis. The finding of alterations in the methylation pattern of genes studied and its association with prognostic factors is a helpful tool in the search for new criteria in clinical and therapeutic decision making.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Stomach Neoplasms
/
Genes, Tumor Suppressor
/
Promoter Regions, Genetic
/
DNA Methylation
Type of study:
Prognostic study
Limits:
Adult
/
Aged
/
Aged, 80 and over
/
Female
/
Humans
/
Male
Country/Region as subject:
South America
/
Chile
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2005
Document type:
Article
/
Project document
Affiliation country:
Chile
Institution/Affiliation country:
Universidad de La Frontera/CL