Automated systems in the identification and determination of methicillin resistance among coagulase negative staphylococci
Mem. Inst. Oswaldo Cruz
; 101(3): 277-280, May 2006. tab
Article
in En
| LILACS
| ID: lil-431726
Responsible library:
BR1.1
RESUMO
Coagulase-negative staphylococci (CoNS) are an important cause of nosocomial bacteremia, specially in patients with indwelling devices or those submitted to invasive medical procedures. The identification of species and the accurate and rapid detection of methicillin resistance are directly dependent on the quality of the identification and susceptibility tests used, either manual or automated. The objective of this study was to evaluate the accuracy of two automated systems MicroScan and Vitek - in the identification of CoNS species and determination of susceptibility to methicillin, considering as gold standard the biochemical tests and the characterization of the mecA gene by polymerase chain reaction, respectively. MicroScan presented better results in the identification of CoNS species (accuracy of 96.8 vs 78.8 percent, respectively); isolates from the following species had no precise identification Staphylococcus haemolyticus, S. simulans, and S. capitis. Both systems were similar in the characterization of methicillin resistance. The higher discrepancies for gene mec detection were observed among species other than S. epidermidis (S. hominis, S. saprophyticus, S. sciuri, S. haemolyticus, S. warneri, S. cohnii), and those with borderline MICs.
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Staphylococcus
/
Bacterial Proteins
/
Microbial Sensitivity Tests
/
Methicillin Resistance
/
Bacterial Typing Techniques
/
Coagulase
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
2006
Document type:
Article
Affiliation country:
Brazil
Country of publication:
Brazil