Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples
Braz. j. infect. dis
; 10(5): 341-345, Oct. 2006. tab
Article
in English
| LILACS, Sec. Est. Saúde SP
| ID: lil-440694
Responsible library:
BR1.1
ABSTRACT
Rapid diagnosis of rabies in suspected human cases influences post-exposure prophylaxis for potential contacts of the patient and ensures appropriate patient management. Apart from the central nervous system (CNS), rabies virus (RABV) is usually present in small sensory nerves adjacent to hair follicles of infected humans. We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this same technique. Results were confirmed by DNA sequencing; this validates RT-PCR and neck-skin as a suitable technique and type of sample, respectively, for use in the diagnosis of human rabies. RT-PCR applied to neck-skin biopsies could allow early diagnosis and lead to more effective rabies treatment.
Full text:
Available
Collection:
National databases
/
Brazil
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
LILACS
/
Sec. Est. Saúde SP
Main subject:
Rabies
/
Rabies virus
/
Skin
/
Neck
Type of study:
Diagnostic study
/
Screening study
Limits:
Animals
/
Humans
Language:
English
Journal:
Braz. j. infect. dis
Year:
2006
Document type:
Article
Institution/Affiliation country:
Hospital Universitário João de Barros Barreto/BR
/
Instituto Evandro Chagas/BR
/
Instituto Pasteu/BR
/
Instituto Pasteur/BR
/
Ministério da Saúde/BR