Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system
Braz. j. microbiol
; 38(1): 62-64, Jan.-Mar. 2007. graf
Article
in English
| LILACS
| ID: lil-449368
Responsible library:
BR32.1
ABSTRACT
A lipase-producing yeast strain isolated from crude cheese and identified as Trichosporon spp produced 7.3 U/mL (59.3 U/µg) after 72h of cultivation. Lipase showed optimum activity at pH 7.0-8.0 and 45-50°C. Extraction by the two-phase aqueous system (PEG-phosphate salts) showed an elevated recuperation (99.8 percent) of enzymatic activity in the PEG phase.
RESUMO
Uma levedura produtora de lipase isolada de queijo coalho e identificada como Trichosporon spp produziu 7,3 U/mL (59,3 U/µg) após 72h de cultivo. A lipase mostrou atividade ótima em pH 7,0-8,0 e temperatura ótima entre 45-50°C. Extração pelo sistema PEG - sais de fosfato apresentou 99,8 por cento de recuperação da atividade enzimática na fase PEG.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
In Vitro Techniques
/
Trichosporon
/
Cheese
/
Clinical Enzyme Tests
/
Lipase
Type of study:
Evaluation study
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2007
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Pernambuco/BR
/
University of Pernambuco/BR