Preservação do sistema genital de chinchila (Chinchilla lanigera) para avaliação em microscopia de luz / Preservation of chinchilla genital system (Chinchilla lanigera) for microscopic evaluation

ABSTRACT

The preservation of chinchilla genital organs using a fixed solution of paraformaldehyde 10 percent buffered and a saturated solution of Bouin during 4, 12 and 18 hours of fixation, inclusion in paraffin and staining with hematoxylin-eosin was evaluated. The Bouin solution with 12 hours of fixation was the best protocol of fixation for the ovaries, oviduct, uterus and vagina, resulting in little tissue retraction and better cellular integration when compared to the other fixing times (4 and 18 hours). The fixation with paraformaldehyde showed good results of tissues fixation. Chinchilla genital organs can be preserved with paraformaldehyde 10 percent buffered and Bouin solution using 12 hours of fixation.