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Ethanol mediated enhancement in bacterial transformation
Sharma, Arun Dev; Singh, Jaspreet; Gill, Prabhjot Kaur.
Affiliation
  • Sharma, Arun Dev; Lyallpur Khalsa College. Department of Biotechnology. Jalandhar. IN
  • Singh, Jaspreet; Lyallpur Khalsa College. Department of Biotechnology. Jalandhar. IN
  • Gill, Prabhjot Kaur; Lovely Institute of Higher Studies. Kapurthala. IN
Electron. j. biotechnol ; 10(1): 166-168, Jan. 2007. ilus, tab
Article in English | LILACS | ID: lil-460055
Responsible library: CL1.1
ABSTRACT
In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10 percent concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications.
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Full text: Available Collection: International databases Database: LILACS Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2007 Document type: Article Affiliation country: India Institution/Affiliation country: Lovely Institute of Higher Studies/IN / Lyallpur Khalsa College/IN
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Full text: Available Collection: International databases Database: LILACS Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2007 Document type: Article Affiliation country: India Institution/Affiliation country: Lovely Institute of Higher Studies/IN / Lyallpur Khalsa College/IN