Ochratoxin A in raisins samples marketed in São Paulo, Brazil
Rev. Inst. Adolfo Lutz
; 65(3): 171-175, set.-dez. 2006. tab, graf
Article
in English
| LILACS, Sec. Est. Saúde SP, SESSP-CTDPROD, Sec. Est. Saúde SP, SESSP-IALPROD, Sec. Est. Saúde SP
| ID: lil-461272
Responsible library:
BR91.2
ABSTRACT
The present study aimed to evaluate a methodology for analyzing ochratoxin A (OTA) in raisins samples marketed in São Paulo, Brazil. OTA was extracted with methanol water (8020, v/v) and diluted with phosphate buffer solution, and purified through an immunoaffinity column. OTA was separated and quantified using HPLC with fluorescence detection. The established detection and quantification limits were 0.24 and 0.80 ng/g, respectively. The recoveries values were 81.6, 80.4 and 81.9%, and the relative standard deviations (RSD) were 6.0, 4.3, and 6.1 % at 2.0, 5.0 and 10.0 ng/g levels, respectively. Of twenty black raisin samples analyzed 10 (50 %) contained OTA at levels ranging from 1.3 to 39.1 ng/g. All of twenty-two white raisin samples showed no contamination with OTA.
Search on Google
Collection:
National databases
/
Brazil
Database:
LILACS
/
Sec. Est. Saúde SP
/
SESSP-CTDPROD
/
SESSP-IALPROD
Main subject:
Brazil
/
Vitis
/
Ochratoxins
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Rev. Inst. Adolfo Lutz
Year:
2006
Document type:
Article
Institution/Affiliation country:
Instituto Adolfo Lutz/BR