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The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle
Zimmermann, Katrin; Holtz, Marlis; Phi-Van, Loc.
Affiliation
  • Zimmermann, Katrin; Federal Agricultural Research Centre. Institute for Animal Welfare and Animal Husbandry. DE
  • Holtz, Marlis; Federal Agricultural Research Centre. Institute for Animal Welfare and Animal Husbandry. DE
  • Phi-Van, Loc; Federal Agricultural Research Centre. Institute for Animal Welfare and Animal Husbandry. DE
Biol. Res ; 40(2): 185-192, 2007. ilus
Article in En | LILACS | ID: lil-468189
Responsible library: BR1.1
ABSTRACT
We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G0 and S phases as well as at the G0/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G0 phase and that this change in chromatin structure was reversible after the cells began to enter S phase.
Subject(s)
Key words
Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Transcription Factors / Chromatin / Cell Cycle / DNA Replication Type of study: Guideline Limits: Humans Language: En Journal: Biol. Res Journal subject: BIOLOGIA Year: 2007 Document type: Article / Project document Affiliation country: Germany Country of publication: Chile
Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Transcription Factors / Chromatin / Cell Cycle / DNA Replication Type of study: Guideline Limits: Humans Language: En Journal: Biol. Res Journal subject: BIOLOGIA Year: 2007 Document type: Article / Project document Affiliation country: Germany Country of publication: Chile