Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
J. venom. anim. toxins incl. trop. dis
; 13(4): 874-880, 2007. ilus
Article
in English
| LILACS
| ID: lil-471147
Responsible library:
BR33.1
ABSTRACT
A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.
Full text:
Available
Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
LILACS
Main subject:
Plasmids
/
Rabies virus
/
Recombinant Fusion Proteins
/
Glycoproteins
/
Transfection
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Journal subject:
Toxicology
Year:
2007
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Pasteur Institute/BR
/
University of São Paulo/BR