An in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulation
Mem. Inst. Oswaldo Cruz
; 102(8): 983-990, Dec. 2007. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-471848
Responsible library:
BR1.1
ABSTRACT
An important cytokine role in dengue fever pathogenesis has been described. These molecules can be associated with haemorrhagic manifestations, coagulation disorders, hypotension and shock, all symptoms implicated in vascular permeability and disease worsening conditions. Several immunological diseases have been treated by cytokine modulation and dexamethasone is utilized clinically to treat pathologies with inflammatory and autoimmune ethiologies. We established an in vitro model with human monocytes infected by dengue virus-2 for evaluating immunomodulatory and antiviral activities of potential pharmaceutical products. Flow cytometry analysis demonstrated significant dengue antigen detection in target cells two days after infection. TNF-alpha, IFN-alpha, IL-6 and IL-10 are produced by in vitro infected monocytes and are significantly detected in cell culture supernatants by multiplex microbead immunoassay. Dexamethasone action was tested for the first time for its modulation in dengue infection, presenting optimistic results in both decreasing cell infection rates and inhibiting TNF-alpha, IFN-alpha and IL-10 production. This model is proposed for novel drug trials yet to be applyed for dengue fever.
Full text:
Available
Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Dengue
/
Neglected Diseases
Database:
LILACS
Main subject:
Dexamethasone
/
Monocytes
/
Cytokines
/
Dengue Virus
/
Glucocorticoids
/
Immunologic Factors
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2007
Document type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Fiocruz/BR