The relation between Escherichia coli surface functional groups' composition and their physicochemical properties
Braz. j. microbiol
; 39(1): 10-15, Jan.-Mar. 2008. ilus, tab
Article
in En
| LILACS
| ID: lil-480664
Responsible library:
BR32.1
ABSTRACT
Escherichia coli surface characteristics including hydrophobicity, electrophoretic mobility and surface functional groups' composition were investigated. These characteristics were determined respectively by water contact angle measurements, microelectrophoresis and x-ray photoelectron spectroscopy (XPS). The relation between the physicochemical properties and functional groups' composition was also examined. The electrophoretic mobility at pH 7 appeared to be governed on the cell surface by the (O=C) functional groups. The cell surface's hydrophilicity was associated with high levels of (C-(O.N)) and (OH-(C-O-C)) functional groups, whereas the cell surface's hydrophobicity was associated with (C-(C,H)) functional groups.
RESUMO
Características de superfície de Escherichia coli, tais como hidrofobicidade, mobilidade eletroforética e composição dos grupos funcionais de superfície, foram estudadas. Essas características foram determinadas por medidas de angulo de contato com água, microeletroforese e espectroscopia fotoeletrônica de raio-X (XPS), respectivamente. A relação entre as propriedades fisicoquímicas e a composição dos grupos funcionais foi também examinada. Aparentemente, a mobilidade eletroforética em pH 7 é controlada na superfície celular pelos grupos funcionais (O=C). A hidrofilicidade da superfície celular estava associada com altos níveis dos grupos funcionais [C-(O.N)] e [OH-(C-O-C)], enquanto a hidrofobicidade estava associada com os grupos funcionais [C-(C,H)].
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Urinary Tract Infections
/
In Vitro Techniques
/
Electrophoresis, Microchip
/
Escherichia coli
/
Hydrophobic and Hydrophilic Interactions
Language:
En
Journal:
Braz. j. microbiol
Journal subject:
MICROBIOLOGIA
Year:
2008
Document type:
Article
Affiliation country:
France
/
Morocco
Country of publication:
Brazil