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The relation between Escherichia coli surface functional groups' composition and their physicochemical properties
Hamadi, Fatima; Latrache, Hassan; Zahir, Hafida; Elghmari, Abderrahmene; Timinouni, Mohamed; Ellouali, Mostapha.
Affiliation
  • Hamadi, Fatima; FST. L'environmental et la Santé. Beni Mellal. MA
  • Latrache, Hassan; FST. L'environmental et la Santé. Beni Mellal. MA
  • Zahir, Hafida; FST. L'environmental et la Santé. Beni Mellal. MA
  • Elghmari, Abderrahmene; FST. Laboratoire de Télédétection et SIG. Beni Mellal. MA
  • Timinouni, Mohamed; Institut Pasteur. Laboratoire de la microbiologie et de la biologie moléculaire. Casablanca. MA
  • Ellouali, Mostapha; FST. L'environmental et la Santé. Beni Mellal. MA
Braz. j. microbiol ; 39(1): 10-15, Jan.-Mar. 2008. ilus, tab
Article in En | LILACS | ID: lil-480664
Responsible library: BR32.1
ABSTRACT
Escherichia coli surface characteristics including hydrophobicity, electrophoretic mobility and surface functional groups' composition were investigated. These characteristics were determined respectively by water contact angle measurements, microelectrophoresis and x-ray photoelectron spectroscopy (XPS). The relation between the physicochemical properties and functional groups' composition was also examined. The electrophoretic mobility at pH 7 appeared to be governed on the cell surface by the (O=C) functional groups. The cell surface's hydrophilicity was associated with high levels of (C-(O.N)) and (OH-(C-O-C)) functional groups, whereas the cell surface's hydrophobicity was associated with (C-(C,H)) functional groups.
RESUMO
Características de superfície de Escherichia coli, tais como hidrofobicidade, mobilidade eletroforética e composição dos grupos funcionais de superfície, foram estudadas. Essas características foram determinadas por medidas de angulo de contato com água, microeletroforese e espectroscopia fotoeletrônica de raio-X (XPS), respectivamente. A relação entre as propriedades fisicoquímicas e a composição dos grupos funcionais foi também examinada. Aparentemente, a mobilidade eletroforética em pH 7 é controlada na superfície celular pelos grupos funcionais (O=C). A hidrofilicidade da superfície celular estava associada com altos níveis dos grupos funcionais [C-(O.N)] e [OH-(C-O-C)], enquanto a hidrofobicidade estava associada com os grupos funcionais [C-(C,H)].
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Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Urinary Tract Infections / In Vitro Techniques / Electrophoresis, Microchip / Escherichia coli / Hydrophobic and Hydrophilic Interactions Language: En Journal: Braz. j. microbiol Journal subject: MICROBIOLOGIA Year: 2008 Document type: Article Affiliation country: France / Morocco Country of publication: Brazil
Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Urinary Tract Infections / In Vitro Techniques / Electrophoresis, Microchip / Escherichia coli / Hydrophobic and Hydrophilic Interactions Language: En Journal: Braz. j. microbiol Journal subject: MICROBIOLOGIA Year: 2008 Document type: Article Affiliation country: France / Morocco Country of publication: Brazil