Your browser doesn't support javascript.
loading
Glycol methacrylate-embedding medium to study morphological alterations of saphenous vein under brief and crescent pressurizations / Inclusão em glicol metacrilato para estudar alterações morfológicas de veias safenas humanas submetidas a pressurizações breves e crescentes
Prado, Cibele M; Viaro, Fernanda; Baldo, Caroline F; Augusto, Viviane dos Santos; Rodrigues, Alfredo José; Evora, Paulo Roberto Barbosa.
Affiliation
  • Prado, Cibele M; University of São Paulo. Ribeirão Preto Faculty of Medicine. Department of Pathology. BR
  • Viaro, Fernanda; University of São Paulo. Ribeirão Preto Faculty of Medicine. Department of Pathology. BR
  • Baldo, Caroline F; University of São Paulo. Ribeirão Preto Faculty of Medicine. Department of Pathology. BR
  • Augusto, Viviane dos Santos; University of São Paulo. Ribeirão Preto Faculty of Medicine. Department of Pathology. BR
  • Rodrigues, Alfredo José; University of São Paulo. Ribeirão Preto Faculty of Medicine. Department of Pathology. Division Thoracic and Cardiovascular Surgery. BR
  • Evora, Paulo Roberto Barbosa; University of São Paulo. Ribeirão Preto Faculty of Medicine. Department of Pathology. Division Thoracic and Cardiovascular Surgery. BR
Acta cir. bras ; 23(supl.1): 77-82, 2008. ilus, graf
Article in English | LILACS | ID: lil-483128
Responsible library: BR1.1
ABSTRACT

PURPOSE:

This study sought to evaluate the efficiency of glycol methacrylate-embedding medium to detect morphological alterations of human saphenous vein submitted to brief and crescent pressurizations.

METHODS:

Saphenous veins of 20 CABG patients were randomly distributed into four experimental groups (control, 100, 200 and 300 mmHg pressures during 15 seconds). To quantify the percentage of endothelium spread over vein surface a microscope magnification of 100x was used for measurements. Morphometric analysis was performed using videomicroscopy with the Leica Qwin software in conjunction with a Leica microscope, videocamera, and an on-line computer.

RESULTS:

A slight tendency of quantitative increase was observed in all parameters including percentage of endothelium spread over vein surface and thickness of saphenous vein walls (intima and media layers).

CONCLUSIONS:

The glycol methacrylate-embedding allowed sections with adequate resolution of structural details and revealed to be an extremely useful method to study pressurized human saphenous veins.
RESUMO

OBJETIVO:

Avaliar a inclusão em glicol metacrilato para estudar alterações morfológicas de veias safenas humanas submetidas a pressurizações breves e crescentes.

MÉTODOS:

Veias safena de 20 pacientes submetidos a cirurgia de revascularização do miocárdio foram distribuídas ao acaso em quatro grupos experimentais (controle, pressões de 100, 200 e 300 mmHg durante 15 segundos). Para quantificar a percentagem da superfície venosa recoberta por endotélio utilizou-se o aumento de 100x. A análise morfométrica foi realizada utilizando-se videomicroscopia com auxílio do software Leica Qwin em conjunto com um microscópio Leica e videocâmera, acoplados a um computador.

RESULTADOS:

Observou-se uma leve tendência de aumento quantitativo de todos os parâmetros avaliados, incluindo a percentagem de superfície recoberta por endotélio e a espessura das paredes das veias safenas.

CONCLUSÕES:

A inclusão em glicol metracrilato permitiu secções com adequada resolução dos detalhes estruturais, revelando-se um método extremamente útil para o estudo de veias safenas humanas pressurizadas.
Subject(s)

Full text: Available Collection: International databases Database: LILACS Main subject: Pressure / Saphenous Vein / Plastic Embedding / Tunica Intima / Methacrylates Type of study: Evaluation study Limits: Humans Language: English Journal: Acta cir. bras Journal subject: CIRURGIA GERAL / Procedimentos Cir£rgicos Operat¢rios Year: 2008 Document type: Article Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR
Full text: Available Collection: International databases Database: LILACS Main subject: Pressure / Saphenous Vein / Plastic Embedding / Tunica Intima / Methacrylates Type of study: Evaluation study Limits: Humans Language: English Journal: Acta cir. bras Journal subject: CIRURGIA GERAL / Procedimentos Cir£rgicos Operat¢rios Year: 2008 Document type: Article Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR
...