Changes in types of muscle fibers induced by transcutaneous electrical stimulation of the diaphragm of rats
Braz. j. med. biol. res
; 41(9): 809-811, Sept. 2008. tab
Article
in English
| LILACS
| ID: lil-492876
Responsible library:
BR1.1
ABSTRACT
The objective of the present study was to assess the effect of transcutaneous electrical diaphragmatic stimulation (TEDS) on different types of diaphragm muscle fibers. Male Wistar rats (8-12 weeks old) were divided into 2 experimental groups (N = 8 in each group) 1) control, 2) animals submitted to TEDS [frequency = 50 Hz; T ON/T OFF (contraction/relaxation time) = 2/2 s; pulse duration = 0.4 ms, intensity = 5 mA with a 1 mA increase every 3 min for 20 min] for 7 days. After completing this treatment period, the I, IIA, IIB, and IID diaphragm muscle fibers were identified using the mATPase technique. Statistical analysis consisted of the normality, homoscedasticity and t-tests (P < 0.05). There was a 19.6 percent (P < 0.05) reduction in the number of type I fibers and a 49.7 percent increase (P < 0.05) in type IID fibers in the TEDS group compared with the control group. An important result of the present study was that electrical stimulation with surface electrodes was efficient in altering the distribution of fibers in diaphragm muscle. This therapeutic resource could be used in the treatment of respiratory muscle alterations.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Diaphragm
/
Transcutaneous Electric Nerve Stimulation
/
Muscle Fibers, Skeletal
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2008
Document type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de São Carlos/BR
/
Universidade Metodista de Piracicaba/BR