Serodiagnosis of tuberculosis: specific detection of free and complex-dissociated antibodies anti-mycobacterium tuberculosis recombinant antigens
Braz. j. infect. dis
; 12(3): 234-244, June 2008. graf, tab
Article
in En
| LILACS
| ID: lil-493654
Responsible library:
BR1.1
ABSTRACT
The diagnostic test characteristics of detecting free and complex-dissociated IgG to three recombinant antigens of Mycobacterium tuberculosis (38-kDa, Ag16 and Ag85B), singly and in combination, were evaluated in sera from 161 tuberculous patients [smear-positive pulmonary TB (50), smear-negative pulmonary TB (pTBsm-) (60) and extrapulmonary TB (51)) and 214 control patients (mycobacteriosis (14), mycoses(14), leprosy(4), other underlying diseases (82) and healthy people (100)]. The individual antigens ranged from 25 percent to 42 percent in sensitivity and from 93 percent to 96 percent in specificity, while considering free IgG response. Addition of complex-dissociated antibodies against each individual antigen improved the sensitivity up to 55 percent. The number and levels of specific antibodies varied greatly from individual to individual. Combination of individual results for free and complex-dissociated IgG to 38-kDa, Ag16 and Ag85B offered 76 percent sensitivity and 83 percent specificity. When the three antigens were placed in the same well, the sensitivity was lower than that expected on the basis of single antigen (63 percent) but with a good specificity (95 percent), even in the group of mycobacteriosis or mycoses. The highest contribution of complex-dissociated IgG results to free IgG results was seen for the diagnosis of pTBsm- patients. In conclusion, although neither single recombinant antigen was reactive with most sera from TB patients even after the measurement of both free and complex-dissociated antibodies, the use of multi-antigen cocktails improved the diagnostic utility of the ELISA assay, allowing the identification of almost 70 percent of pTBsm-, with a high level of specificity; the use of additional, well selected antigens should lead to the detection of almost all patients with TB.
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Tuberculosis
/
Immunoglobulin G
/
Antibodies, Bacterial
/
Mycobacterium tuberculosis
/
Antigen-Antibody Complex
/
Antigens, Bacterial
Type of study:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
En
Journal:
Braz. j. infect. dis
Journal subject:
DOENCAS TRANSMISSIVEIS
Year:
2008
Document type:
Article
Affiliation country:
Argentina
/
Germany
Country of publication:
Brazil