Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
J. appl. oral sci
; 16(5): 340-344, Sept.-Oct. 2008. ilus
Article
in English
| LILACS
| ID: lil-495138
Responsible library:
BR1.1
ABSTRACT
This study investigated the presence of human papillomavirus (HPV) DNA and viral types in 33 cases of oral squamous cells carcinoma (OSCC) and compared the immunohistochemical expression of the cell-cycle markers p21 and pRb between cases of the disease with and without HPV. DNA was extracted from paraffin-embedded tissue and amplified by PCR for the detection of HPV DNA. Viral typing was performed by dot blot hybridization. Immunohistochemistry was performed by the streptavidinbiotin technique. HPV DNA was detected in 11 (33.33 percent) of the 33 cases. The prevalent viral type was HPV 18 (81.81 percent). A significant association was observed between the presence of HPV and immunohistochemical expression of pRb, but not between p21 expression and the presence of the virus. The low frequency of detection of HPV DNA in OSCC suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Mouth Neoplasms
/
Carcinoma, Squamous Cell
/
Retinoblastoma Protein
/
Alphapapillomavirus
Type of study:
Diagnostic study
/
Observational study
/
Risk factors
Limits:
Adult
/
Aged
/
Aged, 80 and over
/
Female
/
Humans
/
Male
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
J. appl. oral sci
Journal subject:
Dentistry
Year:
2008
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Rio Grande do Norte/BR
/
Federal University of Sergipe/BR
/
State University of Feira de Santana/BR